SYBR Green real time PCR for detecting SARS-CoV-2

The SYBR Green I chimeric fluorescence method is a technique used in molecular biology and genetics to detect and quantify DNA or RNA using the fluorescent dye SYBR Green I. 

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SYBR Green I chimeric fluorescence method 

This method can be utilized for the detection of SARS-CoV-2, the virus responsible for COVID-19. It relies on the amplification and detection of specific regions of the SARS-CoV-2 viral genome.

To detect SARS-CoV-2 using the SYBR Green I chimeric fluorescence method, the following steps are typically followed:

1.Primer design:

 Primers are designed to specifically target regions of the SARS-CoV-2 genome that are unique to the virus. These primers will be used to amplify the viral genetic material during the PCR process.

2. RNA extraction: 

Total RNA is extracted from the patient sample, typically from respiratory samples like nasopharyngeal swabs or saliva. This step is crucial for obtaining the viral RNA, which serves as the template for the subsequent PCR amplification.

3. Reverse transcription: 

The extracted RNA is converted into complementary DNA (cDNA) through a reverse transcription reaction. This step involves the use of reverse transcriptase enzymes and primers specific to the viral RNA.

4. PCR amplification: 

The cDNA obtained from the reverse transcription step is then subjected to PCR amplification using the specific primers designed for SARS-CoV-2. The PCR reaction mix includes SYBR Green I dye, which binds to the double-stranded DNA generated during the amplification process.

5. Real-time monitoring: 

The PCR amplification is performed in a real-time PCR instrument, which monitors the fluorescence emitted by the SYBR Green I dye during each amplification cycle. The fluorescence signal increases as the amount of amplified SARS-CoV-2 DNA accumulates.

6. Data analysis: 

The instrument's software analyzes the real-time fluorescence data and provides information on the amplification curve, cycle threshold (Ct) values, and the presence or absence of SARS-CoV-2 based on the specific Ct cutoffs.

Taq Pro Universal SYBR qPCR Master Mix 

This product is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Taq ProDNA Polymerase, is a new generation of hot-start polymerase modified by antibody method.

   Advantagesstrong specificity , high detection sensitivity and  high amplification yield.